Post-Bisulfite DNA Library Preparation Kit (For Illumina®)
Post-Bisulfite DNA Library Preparation Kit (For Illumina®)
Brand : ABCAM
Description
- ab185906 is a complete set of optimized reagents to prepare a DNA library - after successful bisulfite conversion - for various Illumina® platform-based bisulfite sequencing (bisulfite-seq) assays, such as whole genome bisulfite sequencing (WGBS), oxidative bisulfite sequencing (oxBs-seq), reduced representative bisulfite sequencing (RRBS), and other bisulfite-based next generation sequencing applications.
- The optimized protocol and components of the kit allow both non-barcoded (singleplexed) and barcoded (multiplexed) DNA libraries to be quickly constructed using sub-nanogram input concentrations of DNA since the DNA is first bisulfite-converted and then used for library preparation.
Sensitivity
- 1 ng
Range
- 0.5 ng - 1000 ng
Assay time
- 5h 00m
Properties
| Components | 12 tests |
| 10X dA-Tailing Buffer | 1 x 40µl |
| 10X End Repair Buffer | 1 x 40µl |
| 2X HiFi PCR Master Mix | 1 x 160µl |
| 2X Ligation Buffer | 1 x 250µl |
| 5X Conversion Buffer | 1 x 50µl |
| Adaptors (50 μM) | 1 x 15µl |
| Conversion Enzyme Mix | 1 x 15µl |
| Conversion Primer | 1 x 26µl |
| Elution Buffer | 1 x 1ml |
| End Repair Enzyme Mix | 1 x 25µl |
| Klenow Fragment (3’-5’ exo-) | 1 x 15µl |
| MQ Binding Beads | 1 x 1.8ml |
| Primer I (10 μM) | 1 x 15µl |
| Primer U (10 μM) | 1 x 15µl |
| T4 DNA Ligase | 1 x 15µl |
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