Post-Bisulfite DNA Library Preparation Kit (For Illumina®)

Post-Bisulfite DNA Library Preparation Kit (For Illumina®)

Brand : ABCAM

SKU: ab185906-12 Category: Tags: ,


  • ab185906 is a complete set of optimized reagents to prepare a DNA library - after successful bisulfite conversion - for various Illumina® platform-based bisulfite sequencing (bisulfite-seq) assays, such as whole genome bisulfite sequencing (WGBS), oxidative bisulfite sequencing (oxBs-seq), reduced representative bisulfite sequencing (RRBS), and other bisulfite-based next generation sequencing applications.
  • The optimized protocol and components of the kit allow both non-barcoded (singleplexed) and barcoded (multiplexed) DNA libraries to be quickly constructed using sub-nanogram input concentrations of DNA since the DNA is first bisulfite-converted and then used for library preparation.


  • 1 ng


  • 0.5 ng - 1000 ng

Assay time

  • 5h 00m


Components 12 tests
10X dA-Tailing Buffer 1 x 40µl
10X End Repair Buffer 1 x 40µl
2X HiFi PCR Master Mix 1 x 160µl
2X Ligation Buffer 1 x 250µl
5X Conversion Buffer 1 x 50µl
Adaptors (50 μM) 1 x 15µl
Conversion Enzyme Mix 1 x 15µl
Conversion Primer 1 x 26µl
Elution Buffer 1 x 1ml
End Repair Enzyme Mix 1 x 25µl
Klenow Fragment (3’-5’ exo-) 1 x 15µl
MQ Binding Beads 1 x 1.8ml
Primer I (10 μM) 1 x 15µl
Primer U (10 μM) 1 x 15µl
T4 DNA Ligase 1 x 15µl


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