High-Sensitivity DNA Library Preparation Kit (For Illumina®)

High-Sensitivity DNA Library Preparation Kit (For Illumina®)

Brand : ABCAM

SKU: ab185905-12 Category: Tags: ,


  • High Sensitivity DNA Library Preparation KIt (ab185905) is a complete set of optimized reagents to prepare a DNA library from very small amount of samples for use in next-generation sequencing applications.
  • This kit is suitable for preparing a DNA library using sub-nanogram amounts of DNA input for next generation sequencing applications using an Illumina sequencer.
  • These applications include genomic DNA-seq, ChIP-seq, MeDIP/hMeDIP-seq, traditional bisulfite-seq, and targeted re-sequencing.
  • The optimized protocol and components of the kit allow both non-barcoded (singleplexed) and barcoded (multiplexed) DNA libraries to be constructed quickly with reduced bias.

Storage instructions

Store at -20°C. Please refer to protocols.
Components 12 tests
10X End Polishing Buffer 1 x 30µl
2X HiFi PCR Master Mix 1 x 160µl
2X Ligation Buffer 1 x 250µl
Adaptors (50 μM) 1 x 15µl
Elution Buffer 1 x 1ml
End Polishing Enhancer 1 x 13µl
End Polishing Enzyme Mix 1 x 13µl
MQ Binding Beads 1 x 1.6ml
Primer I (10 μM) 1 x 15µl
Primer U (10 μM) 1 x 15µl
T4 DNA Ligase 1 x 15µl


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