10x RIPA Buffer

10x RIPA Buffer

Brand : ABCAM


  • Abcam’s 10X RIPA lysis buffer is an efficient means of cell lysis and protein solubilization for both adherent and suspension cultured mammalian cells. This reagent effectively extracts cytoplasmic, nuclear and membrane proteins. It is compatible with many downstream applications, including SDS-PAGE, Western blot, immunoprecipitation, ELISA and BCA assays.
  • 10X RIPA Buffer may precipitate when stored at + 4ºC. To dissolve crystals, warm briefly at + 37ºC and mix by inversion. After diluting to 1X bring solution to + 4ºC prior to extracting your samples.



  • Liquid

Storage instructions

  • Store at +4°C.

Storage buffer

  • pH: 7.50
  • Constituents: 0.22% Beta glycerophosphate, 10% 4-Nonylphenol, branched, ethoxylated, 0.18% Sodium orthovanadate, 5% Sodium deoxycholate, 0.38% EGTA, 1% Sodium lauryl sulfate, 6.1% Tris, 0.29% EDTA, 8.8% Sodium chloride, 1.12% Sodium pyrophosphate decahydrate

Research areas

  • Kits/ Lysates/ Other
  • Tools and Reagents
  • RIPA



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