High-Sensitivity DNA Library Preparation Kit (For Illumina®)
High-Sensitivity DNA Library Preparation Kit (For Illumina®)
Brand : ABCAM
Description
- High Sensitivity DNA Library Preparation KIt (ab185905) is a complete set of optimized reagents to prepare a DNA library from very small amount of samples for use in next-generation sequencing applications.
- This kit is suitable for preparing a DNA library using sub-nanogram amounts of DNA input for next generation sequencing applications using an Illumina sequencer.
- These applications include genomic DNA-seq, ChIP-seq, MeDIP/hMeDIP-seq, traditional bisulfite-seq, and targeted re-sequencing.
- The optimized protocol and components of the kit allow both non-barcoded (singleplexed) and barcoded (multiplexed) DNA libraries to be constructed quickly with reduced bias.
Storage instructions
Store at -20°C. Please refer to protocols.
| Components | 12 tests |
| 10X End Polishing Buffer | 1 x 30µl |
| 2X HiFi PCR Master Mix | 1 x 160µl |
| 2X Ligation Buffer | 1 x 250µl |
| Adaptors (50 μM) | 1 x 15µl |
| Elution Buffer | 1 x 1ml |
| End Polishing Enhancer | 1 x 13µl |
| End Polishing Enzyme Mix | 1 x 13µl |
| MQ Binding Beads | 1 x 1.6ml |
| Primer I (10 μM) | 1 x 15µl |
| Primer U (10 μM) | 1 x 15µl |
| T4 DNA Ligase | 1 x 15µl |
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