DNA Library Preparation Kit (For Illumina®)
DNA Library Preparation Kit (For Illumina®)
Brand : ABCAM
Description
- The DNA Library Preparation Kit (For Illumina®) (ab185903) is a complete set of optimized reagents to carry out a successful DNA library preparation.
- The kit is suitable for preparing a DNA library for next generation sequencing applications using an Illumina sequencer, which includes genomic DNA-seq, ChIP-seq, MeDIP/hMeDIP-seq, bisulfite-seq, and targeted re-sequencing.
- The optimized protocol and components of the kit allow both non-barcoded (singleplexed) and barcoded (multiplexed) DNA libraries to be constructed quickly with reduced bias.
Properties
| Components | 12 tests |
| 10X dA-Tailing Buffer | 1 x 40µl |
| 10X End Repair Buffer | 1 x 40µl |
| 2X HiFi PCR Master Mix | 1 x 160µl |
| 2X Ligation Buffer | 1 x 250µl |
| Adaptors (50 μM) | 1 x 15µl |
| Elution Buffer | 1 x 1ml |
| End Repair Enzyme Mix | 1 x 25µl |
| Klenow Fragment (3’-5’ exo-) | 1 x 15µl |
| MQ Binding Beads | 1 x 1.6ml |
| Primer I (10 μM) | 1 x 15µl |
| Primer U (10 μM) | 1 x 15µl |
| T4 DNA Ligase | 1 x 15µl |
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